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DNA sequencing of Arabidopsis thaliana nrpd1-3 background with novel insertions of LTR retrotransposon ONSEN/COPIA78.

ABSTRACT: The intent of the experiment was to infer, from re-sequencing of genomic DNA, the coordinates of neo-insertions from activated ONSEN/COPIA78 LTR retrotrasnposon in Arabidopsis thaliana. For this, we performed Illumina 75 bp pair-end PCR-free DNA genome re-sequencing in several independent lines of RdDM mutant nrpd1-3.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Diego Sanchez 

PROVIDER: E-MTAB-5883 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Developmental Restriction of Retrotransposition Activated in <i>Arabidopsis</i> by Environmental Stress.

Gaubert Hervé H   Sanchez Diego H DH   Drost Hajk-Georg HG   Paszkowski Jerzy J  

Genetics 20170803 2

Retrotransposons (RTs) can rapidly increase in copy number due to periodic bursts of transposition. Such bursts are mutagenic and thus potentially deleterious. However, certain transposition-induced gain-of-function or regulatory mutations may be of selective advantage. How an optimal balance between these opposing effects arises is not well characterized. Here, we studied transposition bursts of a heat-activated retrotransposon family in <i>Arabidopsis</i> We recorded a high inter and intraplan  ...[more]

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