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RNA-seq of definitive endoderm and pancreatic progenitor cells derived in vitro from human pluripotent stem cells in order to study the role of transcription factor GATA6 in human pancreas development

ABSTRACT: Mutant GATA6 hPSCs were obtained by TALEN genome editing or re-programmed from patient fibroblasts. Along with wild-type H9 cells, these GATA6 mutant cell lines were differentiated into SOX17+/CXCR4+ endodermal cells (day 3) and PDX1+ pancreatic progenitor cells (day 12). The purpose of this work was to study the role of GATA6 in the development of the human pancreas at a molecular level.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Ray Dunn

PROVIDER: E-MTAB-5958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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GATA6 Cooperates with EOMES/SMAD2/3 to Deploy the Gene Regulatory Network Governing Human Definitive Endoderm and Pancreas Formation.

Chia Crystal Y CY Madrigal Pedro P Denil Simon L I J SLIJ Martinez Iker I Garcia-Bernardo Jose J El-Khairi Ranna R Chhatriwala Mariya M Shepherd Maggie H MH Hattersley Andrew T AT Dunn N Ray NR Vallier Ludovic L

Stem cell reports 20190101 1

Heterozygous de novo mutations in GATA6 are the most frequent cause of pancreatic agenesis in humans. In mice, however, a similar phenotype requires the biallelic loss of Gata6 and its paralog Gata4. To elaborate the human-specific requirements for GATA6, we chose to model GATA6 loss in vitro by combining both gene-edited and patient-derived pluripotent stem cells (hPSCs) and directed differentiation toward β-like cells. We find that GATA6 heterozygous hPSCs show a modest reduction in definitive ...[more]

PMID: 30629940

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