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ScRNA-seq to investigate endoderm differentiation from induced human pluripotent stem cells

ABSTRACT: Single-cell RNA-seq (scRNA-seq) on nocodazole and DMSO treated cells before and after differentiation into endoderm. hPSC colonies were treated with DMSO or 100ng/ml nocodazole for 16 hours and induced to differentiate into definitive endoderm for three days. Single cells were subsequently collected in either undifferentiated conditions (Und) or after 3 days of endoderm differentiation (Endoderm) and then sorted onto 384 well plates for Smart-Seq2 processing.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Daniele Muraro

PROVIDER: E-MTAB-7008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics.

Yiangou Loukia L Grandy Rodrigo A RA Morell Carola M CM Tomaz Rute A RA Osnato Anna A Kadiwala Juned J Muraro Daniele D Garcia-Bernardo Jose J Nakanoh Shota S Bernard William G WG Ortmann Daniel D McCarthy Davis J DJ Simonic Ingrid I Sinha Sanjay S Vallier Ludovic L

Stem cell reports 20181227 1

Cell cycle progression and cell fate decisions are closely linked in human pluripotent stem cells (hPSCs). However, the study of these interplays at the molecular level remains challenging due to the lack of efficient methods allowing cell cycle synchronization of large quantities of cells. Here, we screened inhibitors of cell cycle progression and identified nocodazole as the most efficient small molecule to synchronize hPSCs in the G2/M phase. Following nocodazole treatment, hPSCs remain pluri ...[more]

PMID: 30595546

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