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Intestinal Fork Head Regulates Nutrient Absorption and Promotes Longevity

ABSTRACT: We identified that Drosophila FoxA transcription Fork Head (FKH) function is necessary for reduced insulin signalling and rapamycin induced longevity. Furthermore, we determined that FKH function in the gut tissue alone is sufficient to extend lifespan. We carried out RNA-sequencing in gut tissue on controls (daGS;InRDN>GFP- RNAi) and reduced insulin signalling background upon control RNAi (daGS;InRDN>GFPRNAi +RU486) and upon FKH RNAi (daGS;InRDN>FKH- RNAi +RU486). InR-DN stock used: K1409A.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ekin Bolukbasi

PROVIDER: E-MTAB-6056 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Intestinal Fork Head Regulates Nutrient Absorption and Promotes Longevity.

Bolukbasi Ekin E Khericha Mobina M Regan Jennifer C JC Ivanov Dobril K DK Adcott Jennifer J Dyson Miranda C MC Nespital Tobias T Thornton Janet M JM Alic Nazif N Partridge Linda L

Cell reports 20171001 3

Reduced activity of nutrient-sensing signaling networks can extend organismal lifespan, yet the underlying biology remains unclear. We show that the anti-aging effects of rapamycin and reduced intestinal insulin/insulin growth factor (IGF) signaling (IIS) require the Drosophila FoxA transcription factor homolog Fork Head (FKH). Intestinal FKH induction extends lifespan, highlighting a role for the gut. FKH binds to and is phosphorylated by AKT and Target of Rapamycin. Gut-specific FKH upregulati ...[more]

PMID: 29045833

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