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Single-cell RNA-seq of CD4+ Treg and Tmem cells from different tissues in mouse and human

ABSTRACT: Non-lymphoid tissues (NLTs) harbour a pool of adaptive immune cells distinct from their counterparts in lymphoid tissues, and their development and phenotype remains largely unexplored. We used scRNA-seq to survey CD4+ T regulatory (Treg) and memory T (Tmem) cells in spleen, lymph nodes, skin and colon in an unbiased way, in mouse and human. This cross-tissues, cross-species comparison allows us to obtain marker genes for immune populations in specific locations with likely relevance for human studies. Additionally, a continuous phenotype of Treg migration can be modelled from the mouse data, unravelling the transcriptional stages through which these cells transition between tissues.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Tomas Gomes

PROVIDER: E-MTAB-6072 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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