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Single-cell ATAC-seq of a mixture of three oesophageal tissue (HET1A, OE33, OE19) derived cell lines

ABSTRACT: Three oesophageal tissue derived cell lines, one from a normal tissue (HET1A) and two from tumour tissues (OE33 and OE199) were mixed with same number of each cell type in the same tube to get a mixed population. The C1 platform (Fluidigm) was used to capture single-cells and scATAC-seq protocols from Fluidigm ScriptHub is then used to generate the sequencing library. A single-cell ATAC-seq Bioinformatics pipeline is then developed to deconvolute the cells into their respective cell types.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Syed Murtuza Baker

PROVIDER: E-MTAB-6116 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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