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Determination by ChIP-chip of the binding sites of the developmental transcription regulator BldC in the genome of Streptomyces venezuelae ATCC 10712

ABSTRACT: BldC is a transcriptional regulator essential for morphological development Streptomyces venezuelae. Although bldC deletion strain is unable to produce aerial hyphae, electron microscopy reveals that almost all of the colony biomass is in the form of spores rather than undifferentiated vegetative hyphae. This ChIP-chip experiment was carried out to determine the binding sites, and thence the regulon, of BldC in Streptomyces venezuelae. Cy3(IP):Cy5(Total) signal ratios in the wild type were compared to those in a bldC knockout strain.

INSTRUMENT(S): Agilent Technologies G2505C dual laser scanner, Agilent Technologies hybridisation chamber and Agilent Technologies hybridisation oven

ORGANISM(S): Streptomyces venezuelae

SUBMITTER: Matthew Bush

PROVIDER: E-MTAB-6129 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification by ChIP-Seq of the binding sites of the developmental protein BldC in the genome of Streptomyces venezuelae

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