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Identification by ChIP-Seq of the binding sites of the developmental protein BldC in the genome of Streptomyces venezuelae

ABSTRACT: The BldC gene is required for the formation of aerial hyphae Streptomyces venezuelae. It is 68 amino acid DNA-binding protein related to the MerR family of transcription factors. BldC deletion strains are bald because they initiate sporulation prematurely, omitting the formation of aerial hyphae altogether. This ChIP-Seq experiment was carried out to determine all the binding sites BldC binds to in the genome of Streptomyces venezuelae. Anti-BldC antibodies were used for ChIP-Seq of the wild type (WT) strain after 10 and 14 hours of growth in shaken cultures. A BldC deletion strain was made and anti-BldC antibodies were used for ChIP-Seq in the deletion strain after 14 hours of growth in shaken cultures. This was used as the negative control and enrichment in this ChIP-Seq was subtracted from the enrichment in the WT strain.

INSTRUMENT(S): Illumina HiSeq 4000, Dell PowerEdge 630 with Intel Xeon E5-2630 processor (8 cores 16 threads) and 94 GB memory running CentOS Linux release 7.5.1804.

ORGANISM(S): Streptomyces venezuelae

SUBMITTER: Mahmoud Al-Bassam

PROVIDER: E-MTAB-7450 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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