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Transcriptomic characterization of the human cell cycle in individual unsynchronized cells

ABSTRACT: Here, we profiled transcription across the cell cycle in single human myxoid liposarcoma cells, without prior synchronization. Flourescence-activated cell sorting was used to infer G1, S or G2/M cell cycle stages after staining cells for DNA content. Single-cell RNA-sequencing was performed according to the Smart-seq2 protocol.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Erik Larsson Lekholm

PROVIDER: E-MTAB-6142 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic Characterization of the Human Cell Cycle in Individual Unsynchronized Cells.

Karlsson Joakim J Kroneis Thomas T Jonasson Emma E Larsson Erik E Ståhlberg Anders A

Journal of molecular biology 20171016 24

The highly fine-tuned dynamics of cell cycle gene expression have been intensely studied for several decades. However, some previous observations may be difficult to fully decouple from artifacts induced by traditional cell synchronization procedures. In addition, bulk cell measurements may have disguised intricate details. Here, we address this by sorting and transcriptomic sequencing of single cells progressing through the cell cycle without prior synchronization. Genes and pathways with known ...[more]

PMID: 29045817

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