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Whole-Genome Gene Expression Bead Array analysis of Toll-like receptor 2/8 stimulated human monocytes

ABSTRACT: Monocytes and macrophages are essential cells in the early response in their capacity as ubiquitous phagocytic cells. They phagocytose microorganisms or damaged cells and sense pathogen/damage-associated molecular patterns (PAMPs/DAMPs) through innate receptors such as Toll-like receptors (TLRs). We investigated a phenomenon where co-signaling from TLR2 and TLR8 in human primary monocytes provides a distinct immune activation profile compared to signaling from either TLR alone. Comparison of gene signatures induced by either stimulus alone or together, show that co-signaling result in downstream differences in regulation of signaling and gene transcription. To investigate the interaction of TLR2 and TLR8 stimulation primary human monocytes isolated from buffy coat were stimulated with TLR2/6 ligand FSL-1 and/or TLR7/8 ligand CL075 and the gene expression monitored at 1,2 and 3h post stimulation.

ORGANISM(S): Homo sapiens

SUBMITTER: Bjarte Bergstrøm

PROVIDER: E-MTAB-6222 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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