Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq of estrogen (E2) induced changes of mRNA expression in early pregnant Sus scrofa endometrium

ABSTRACT: Maternal exposure to estrogens can induce long-term adverse effects in the offspring. This may be mediated through alterations in the endometrium affecting embryo-maternal communication as early as the preimplantational phase. Thus, we analyzed the effects of gestational estradiol-17β (E2) exposure on the endometrium. Two distinct low doses and a high dose (0.05, 10 and 1000 µg E2/kg body weight daily, respectively) were orally applied to sows from insemination until sampling at day 10 of pregnancy and compared to carrier-treated controls. RNA-sequencing revealed a dose-dependent increase of 14, 17 and 27 differentially expressed genes (DEG), respectively. Overall, the maternal E2 treatment perturbed gene expression of the endometrium, potentially altering the uterine histotroph.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Sus scrofa

SUBMITTER: Stefan Bauersachs

PROVIDER: E-MTAB-6242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exposure of pregnant sows to low doses of estradiol-17β impacts on the transcriptome of the endometrium and the female preimplantation embryos†.

Flöter Veronika L VL Bauersachs Stefan S Fürst Rainer W RW Krebs Stefan S Blum Helmut H Reichenbach Myriam M Ulbrich Susanne E SE

Biology of reproduction 20190301 3

Maternal exposure to estrogens can induce long-term adverse effects in the offspring. The epigenetic programming may start as early as the period of preimplantation development. We analyzed the effects of gestational estradiol-17β (E2) exposure with two distinct low doses, corresponding to the acceptable daily intake "ADI" and close to the no-observed-effect level "NOEL", and a high dose (0.05, 10, and 1000 μg E2/kg body weight daily, respectively). The E2 doses were orally applied to sows from ...[more]

PMID: 30260370

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Dataset Information

RNA-seq of estrogen (E2) induced changes of mRNA expression in early pregnant Sus scrofa endometrium

Publications

Exposure of pregnant sows to low doses of estradiol-17β impacts on the transcriptome of the endometrium and the female preimplantation embryos†.

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