RNA-seq of estrogen (E2) induced changes of mRNA expression in Sus scrofa male and female blastocysts derived from embryonic day 10

ABSTRACT: Environmental estrogens may affect epigenetic programming as early as the period of preimplantation development. Therefore, we analyzed the effects of continuous gestational estradiol-17β (E2) exposure on male and female embryos. A low dose, close to the no-observed effect level (NOEL - 10 µg E2/kg body weight(bw)/d), a high dose (1000 µg E2/kg bw/d) and carrier only, as control group, was fed to sows from insemination until sampling at day 10 of pregnancy, respectively. 36 samples (n = 5-7 per treatment group and sex) were analyzed by high throughput sequencing.In the high dose group, RNA-sequencing of single embryos revealed 982 differentially expressed genes (DEG) in the female but none in the male blastocysts. Moreover, 62 and 3 DEG were found in female and male embryos of the NOEL dos

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Sus scrofa

SUBMITTER: Stefan Bauersachs 

PROVIDER: E-MTAB-6263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress