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Unknown,Transcriptomics,Genomics,Proteomics

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Down-regulatory responses to heat stress and lipopolysaccharide in the chicken thymic transcriptome

ABSTRACT: For this study, thymic transcriptome responses to an acute heat stress and/or lipopolysaccharide (LPS) were investigated in a broiler line (heat and disease susceptible) and an inbred Fayoumi line (heat and disease resistant) of chickens. In a 2 x 2 design, 22 day-old birds were exposed to heat stress (35°C for 7 hours), lipopolysaccharide (100 µg/kg average body weight per line), or both stressors. Thermoneutral temperature (25°C) and phosphate buffered saline were used as the respective controls. Tissue samples were collected from the thymus and used to isolate high quality RNA. cDNA libraries (n = 31) were constructed and sequenced on the HiSeq 2500.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Gallus gallus

SUBMITTER: Melissa Monson

PROVIDER: E-MTAB-6290 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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