Comparison of gene expression in adult and larvae Drosophila melanogaster strains modified with tTAV-based positive feedback loop

ABSTRACT: Synthetic systems that use positive feedback have been developed to control human disease vectors and crop pests. The tTAV system, which has been deployed in several insect species, relies on a positive feedback circuit that can be inhibited via dietary tetracycline. Although insects carrying tTAV fail to survive until adulthood in the absence of tetracycline, the exact reason for its lethality, as well as the transcriptomic effects of an active positive feedback circuit, remain unknown. Understanding what factors contribute to the variance in tTAV-associated mortality is likely to inform the development of insect control systems. In the present study, the OXI513a tTAV feedback circuit was introduced and verified into D. melanogaster. The tight tetracycline regulation of the system affords

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jaroslaw Bryk 

PROVIDER: E-MTAB-6332 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress