Unknown,Transcriptomics,Genomics,Proteomics

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Plasmodium yoelii RNA-Seq


ABSTRACT: Plasmodium yoelii YM asexual blood stage parasites express multiple members of the py235 gene family, part of the super-family of genes including those coding for Plasmodium vivax reticulocyte binding proteins and Plasmodium falciparum RH proteins. Dr Tony Holder's laboratory (NIMR, London) has been successful in deleting one of the RH family genes (Py01365) by transfection and insertion of the TgDHFR gene, and cloned the resulting parasite in YM background. The gene expression patterns of the mutant parasite line were compared to that of the wild type YM parasite.

ORGANISM(S): Plasmodium yoelii

SUBMITTER: Submission Service 

PROVIDER: E-MTAB-636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Targeted disruption of py235ebp-1: invasion of erythrocytes by Plasmodium yoelii using an alternative Py235 erythrocyte binding protein.

Ogun Solabomi A SA   Tewari Rita R   Otto Thomas D TD   Howell Steven A SA   Knuepfer Ellen E   Cunningham Deirdre A DA   Xu Zhengyao Z   Pain Arnab A   Holder Anthony A AA  

PLoS pathogens 20110217 2


Plasmodium yoelii YM asexual blood stage parasites express multiple members of the py235 gene family, part of the super-family of genes including those coding for Plasmodium vivax reticulocyte binding proteins and Plasmodium falciparum RH proteins. We previously identified a Py235 erythrocyte binding protein (Py235EBP-1, encoded by the PY01365 gene) that is recognized by protective mAb 25.77. Proteins recognized by a second protective mAb 25.37 have been identified by mass spectrometry and are e  ...[more]

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