Transcriptional profiling of long-term stimulated primary murine intestinal fibroblasts with interleukin-36.
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ABSTRACT: Previous stimulation experiments of stimulated primary murine colonic fibroblasts with IL-36R ligands for 4h in vitro showed an upregulation of pro-inflammatory cytokines e.g. IL-6, IL-1b and chemokines e.g. CXCL1, CCL2 indicating an important role for IL-36 in inflammation. We were interested in analyzing long-term stimulation (9 days) of intestinal fibroblasts to identify a putative differential expression profile. The experimental setup included 6 samples from 3 colons in a pairwise design (3 stimulated vs 3 unstimulated).
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Mus musculus
SUBMITTER: Clemens Neufert
PROVIDER: E-MTAB-6476 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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