Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Microarray analysis of human umbilical vein endothelial cells (HUVECs) chronically irradiated with low dose-rate of ionizing radiation for one, three and six weeks.

ABSTRACT: To obtain more insight in the molecular effects of chronic low dose irradiation on endothelial cells, HUVEC were irradiated with low-dose rate irradiation (137Cs gamma source; 0, 1.4 and 4.1 mGy/h) for several weeks. At different time points (1, 3 and 6 weeks) cells were harvested for RNA extraction and hybridisation to Affymetrix GeneChip Human Gene 1.0 ST arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Roel Quintens

PROVIDER: E-MTAB-6521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptomic profiling suggests a role for IGFBP5 in premature senescence of endothelial cells after chronic low dose rate irradiation.

Rombouts Charlotte C Aerts An A Quintens Roel R Baselet Bjorn B El-Saghire Hussein H Harms-Ringdahl Mats M Haghdoost Siamak S Janssen Ann A Michaux Arlette A Yentrapalli Ramesh R Benotmane Mohammed Abderrafi MA Van Oostveldt Patrick P Baatout Sarah S

International journal of radiation biology 20140701 7

<h4>Purpose</h4>Ionizing radiation has been recognized to increase the risk of cardiovascular diseases (CVD). However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed.<h4>Material and methods</h4>Previously, human umbilical vein endothelial cells (HUVEC) were exposed to chronic low dose rate radiation (1.4 and 4.1 mGy/h) during one, three and six weeks which resulted in premature senescence in cells ...[more]

PMID: 24646080

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Project description:The growing number of particle treatment facilities worldwide and patients treated with particles instead of X-rays marks the upcoming rearrangement of modern radiotherapy. Especially for tumors being difficult to access and for tumors that are resistant to conventional X-ray treatment particle radiotherapy is a beneficial technology. At the Heidelberg Ion Beam Therapy Center (HIT) patients are treated with this technology since 2009 as it offers clear benefits. Contrary to X-rays, which show an exponential dose decrease (after reaching electron equilibrium) with increasing tissue depth, charged particles deposit most of their energy to a small region within the tissue with a sharp dose fall-off after the so-called Bragg peak. This precise dose localization enables further dose escalation within the tumor while sparing healthy tissue. Besides physical advantages, particle radiotherapy offers additional biological advantages. In radiobiology, the term relative-biological effectiveness (RBE) is defined as the ratio of X-rays dose to an alternative irradiation modality dose which produce the same biological effect (e.g. survival or number of DSBs). While protons have a relative biological effectiveness (RBE) comparable to X-rays, carbon ions are more effective in inducing DNA damage(1, 2) and are therefore especially useful for radioresistant tumors. This is due to the fact, that carbon ions induce clustered and direct DNA damage, which is considered to be less dependent on cell cycle stage, oxygen level, genetic background and hinders DNA repair mechanisms(3–6). Nevertheless, their exact mode of action and cellular mechanisms are largely unknown. We show the first comprehensive proteomic and phosphoproteomic study elucidating the cellular response to treatment with protons, carbon ions and X-rays. We found that 2h after treatment with these radiations negligible regulation occurred at protein expression level. But 181 phosphorylation sites were deregulated by ionizing radiation contributing mainly to DNA damage response functionalities. Interestingly we found 55 phosphorylation sites being differentially regulated between the radiations. Here, we observed protons and carbon ions producing equal cellular response whereas X-rays show altered regulation for certain phosphorylation sites. A subset of 28 phosphorylation sites being involved in the DNA damage response or differentially regulated between the ionizing radiations was selected for result confirmation.

Dataset Information

Microarray analysis of human umbilical vein endothelial cells (HUVECs) chronically irradiated with low dose-rate of ionizing radiation for one, three and six weeks.

Publications

Transcriptomic profiling suggests a role for IGFBP5 in premature senescence of endothelial cells after chronic low dose rate irradiation.

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