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In depth mapping of DNA-PKcs and ATM kinase signaling responses to double strand breaks.

ABSTRACT: In depth analysis of double strand break signaling was performed using mouse Pre-B cells and Human HCT116 cells. Our analysis included the induction of double strand breaks with ionizing radiation with the addition of ATM and DNA-PKcs inhibitors, alone and in combination.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): B Cell, Cell Suspension Culture, Cell Culture

SUBMITTER: Shannon Marshall

LAB HEAD: Marcus Smolka

PROVIDER: PXD042258 | Pride | 2024-10-17

REPOSITORIES: Pride

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			Action	DRS
	062221_HCT116_IR_No_PKi_L_vs_PKi_H_input_1pct.mzXML	Mzxml
	062221_HCT116_IR_No_PKi_L_vs_PKi_H_input_1pct.pep.xml	Pepxml
	062221_HCT116_IR_No_PKi_L_vs_PKi_H_input_1pct.raw	Raw
	062221_HCT116_IR_PKi_L_vs_No_PKi_H_input_1pct.mzXML	Mzxml
	062221_HCT116_IR_PKi_L_vs_No_PKi_H_input_1pct.pep.xml	Pepxml

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Publications

In-depth mapping of DNA-PKcs signaling uncovers noncanonical features of its kinase specificity.

Marshall Shannon S Navarro Marcos V A S MVAS Ascenҫão Carolline F R CFR Dibitetto Diego D Smolka Marcus B MB

The Journal of biological chemistry 20240628 8

DNA-PKcs is a DNA damage sensor kinase with established roles in DNA double-strand break repair via nonhomologous end joining. Recent studies have revealed additional roles of DNA-PKcs in the regulation of transcription, translation, and DNA replication. However, the substrates through which DNA-PKcs regulates these processes remain largely undefined. Here, we utilized quantitative phosphoproteomics to generate a high coverage map of DNA-PKcs signaling in response to ionizing radiation and mappe ...[more]

PMID: 38945450

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