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The effect of the Autoimmune regulator (Aire) gene on mRNA and lncRNA transcriptional profile of mTEC 3.10 cell line

ABSTRACT: In this study, we used the murine (Mus musculus) medullary thymic epithelial cell line (mTEC 3.10 cell line) co-cultured with fresh thymocytes as a functional assay for mTEC-thymocyte adhesion. Then we analyzed the differential transcriptional profile of this cell line, by means of Agilent oligo microarray hybridization, comparing Autoimmune regulator (Aire) wild-type cells vs Crispr-Cas9-induced Aire KO cells. The comparative transcriptional expression signatures allowed us to find those differentially expressed mRNAs or lncRNAs between the samples tested.

ORGANISM(S): Mus musculus

SUBMITTER: Max Jordan Duarte

PROVIDER: E-MTAB-6541 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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