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RNA-Seq of human aortic endothelial cells with lysophosphatidylcholine, IL-35, and IL-10 against controls

ABSTRACT: Human aortic endothelial cells were stimulated by lysophosphatidylcholine (LPC) (10μM) with or without interleukin 35 (IL-35) (10ng/mL) or IL-10 (10ng/mL) for 18 hours. Total RNAs were extracted from samples, then mRNA and non-coding RNAs were enriched by removing rRNA from the total RNA. The library was sequenced by Illumina HiSeq4000 using PE100 strategy and the reads were mapped to the human hg19 reference genome.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Xinyuan Li

PROVIDER: E-MTAB-6604 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lysophospholipids induce innate immune transdifferentiation of endothelial cells, resulting in prolonged endothelial activation.

Li Xinyuan X Wang Luqiao L Fang Pu P Sun Yu Y Jiang Xiaohua X Wang Hong H Yang Xiao-Feng XF

The Journal of biological chemistry 20180516 28

Innate immune cells express danger-associated molecular pattern (DAMP) receptors, T-cell costimulation/coinhibition receptors, and major histocompatibility complex II (MHC-II). We have recently proposed that endothelial cells can serve as innate immune cells, but the molecular mechanisms involved still await discovery. Here, we investigated whether human aortic endothelial cells (HAECs) could be transdifferentiated into innate immune cells by exposing them to hyperlipidemia-up-regulated DAMP mol ...[more]

PMID: 29769317

Publication: 1/2

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