Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA seq of fluid shear stress treated mouse Pkd1-/- renal epithelial cells

ABSTRACT: Pkd1-/- renal epithelial cells are exposed to mechanical forces due to flow-induced shear stress within the nephrons. We applied RNA sequencing to get a comprehensive overview of fluid-shear regulated genes and pathways in the immortalized Pkd1-/- renal proximal tubular epithelial cell line. Cells were exposed to laminar fluid shear stress (1.9 dyn/cm2) in a cone-plate device and compared to static controls.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Steven Kunnen

PROVIDER: E-MTAB-6641 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells.

Kunnen Steven J SJ Malas Tareq B TB Semeins Cornelis M CM Bakker Astrid D AD Peters Dorien J M DJM

Journal of cellular physiology 20171120 4

Renal epithelial cells are exposed to mechanical forces due to flow-induced shear stress within the nephrons. Shear stress is altered in renal diseases caused by tubular dilation, obstruction, and hyperfiltration, which occur to compensate for lost nephrons. Fundamental in regulation of shear stress are primary cilia and other mechano-sensors, and defects in cilia formation and function have profound effects on development and physiology of kidneys and other organs. We applied RNA sequencing to ...[more]

PMID: 29044509

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Dataset Information

RNA seq of fluid shear stress treated mouse Pkd1-/- renal epithelial cells

Publications

Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells.

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