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Transcriptional profiling of Dicer conditional knockout hematopoietic stem cells.

ABSTRACT: Gene expression of HSC (Lin-Sca-Kit+Flt3-) hematopoietic stem cells from Dicer D/+ mice was compared with LT-HSC from DicerD/D mice. BM cells were noncompetitively transplanted into irradiant recipients, and deletion induced by poly(IC) injection. Donor-derived HSCs were isolated by FACS sorting of recipient bone marrow. Biological replicates of each (2 per genotype) were generated and expression profiles were determined by hybridization to Affymetrix Moe430_2 arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Claus Nerlov

PROVIDER: E-MTAB-674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dicer is selectively important for the earliest stages of erythroid development.

Buza-Vidas Natalija N Cismasiu Valeriu B VB Moore Susan S Mead Adam J AJ Woll Petter S PS Lutteropp Michael M Melchiori Luca L Luc Sidinh S Bouriez-Jones Tiphaine T Atkinson Deborah D O'Carroll Donal D Jacobsen Sten Eirik W SE Nerlov Claus C

Blood 20120806 12

MicroRNAs (miRs) are involved in many aspects of normal and malignant hematopoiesis, including hematopoietic stem cell (HSC) self-renewal, proliferation, and terminal differentiation. However, a role for miRs in the generation of the earliest stages of lineage committed progenitors from HSCs has not been identified. Using Dicer inactivation, we show that the miR complex is not only essential for HSC maintenance but is specifically required for their erythroid programming and subsequent generatio ...[more]

PMID: 22869792

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