Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling by high throughput of Blumeria gramis during infection of Barley

ABSTRACT: The aim of this experiment was to investigate the transcriptomic variation within Blumeria graminis f. sp. hordei during infection of Barley. SOLiD RNA-seq data was generated for 6 samples, three from the epiphytic hyphae and three from the haustoria within the epidermis.

ORGANISM(S): Blumeria graminis

SUBMITTER: Spanu Pietro

PROVIDER: E-MTAB-682 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in extreme parasitism.

Spanu Pietro D PD Abbott James C JC Amselem Joelle J Burgis Timothy A TA Soanes Darren M DM Stüber Kurt K Ver Loren van Themaat Emiel E Brown James K M JK Butcher Sarah A SA Gurr Sarah J SJ Lebrun Marc-Henri MH Ridout Christopher J CJ Schulze-Lefert Paul P Talbot Nicholas J NJ Ahmadinejad Nahal N Ametz Christian C Barton Geraint R GR Benjdia Mariam M Bidzinski Przemyslaw P Bindschedler Laurence V LV Both Maike M Brewer Marin T MT Cadle-Davidson Lance L Cadle-Davidson Molly M MM Collemare Jerome J Cramer Rainer R Frenkel Omer O Godfrey Dale D Harriman James J Hoede Claire C King Brian C BC Klages Sven S Kleemann Jochen J Knoll Daniela D Koti Prasanna S PS Kreplak Jonathan J López-Ruiz Francisco J FJ Lu Xunli X Maekawa Takaki T Mahanil Siraprapa S Micali Cristina C Milgroom Michael G MG Montana Giovanni G Noir Sandra S O'Connell Richard J RJ Oberhaensli Simone S Parlange Francis F Pedersen Carsten C Quesneville Hadi H Reinhardt Richard R Rott Matthias M Sacristán Soledad S Schmidt Sarah M SM Schön Moritz M Skamnioti Pari P Sommer Hans H Stephens Amber A Takahara Hiroyuki H Thordal-Christensen Hans H Vigouroux Marielle M Wessling Ralf R Wicker Thomas T Panstruga Ralph R

Science (New York, N.Y.) 20101201 6010

Powdery mildews are phytopathogens whose growth and reproduction are entirely dependent on living plant cells. The molecular basis of this life-style, obligate biotrophy, remains unknown. We present the genome analysis of barley powdery mildew, Blumeria graminis f.sp. hordei (Blumeria), as well as a comparison with the analysis of two powdery mildews pathogenic on dicotyledonous plants. These genomes display massive retrotransposon proliferation, genome-size expansion, and gene losses. The missi ...[more]

PMID: 21148392

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Project description:Even though cutaneous atrophy is the major adverse effect of topical glucocorticoids, its molecular mechanisms are poorly understood. We found that glucocorticoids strongly increased the expression of REDD1 (regulated in development and DNA damage response 1), a stress-inducible inhibitor of mTOR, in mouse and human epidermis. We found that REDD1 knockout animals are partially resistant to glucocorticoid-induced epidermal and subcutaneous adipose atrophy which correlated with the protection of CD34+ follicular epithelial stem cells as well as p63+ keratinocyte progenitors in REDD1 knockout epidermis during chronic steroid treatment. At the same time, REDD1 knockout did not affect anti- inflammatory effect of glucocorticoids, as evaluated by ear edema test. Expression profiling revealed that ~ 50% of the glucocorticoid receptor (GR) target genes were not activated in epidermis of REDD1 knockouts, however, the negative effect of glucocorticoids on gene expression was similar to that in w.t. animals. Overall, our findings reveal a novel pathway in GR activation by its target gene/protein REDD1; and indicate an important role of REDD1 in glucocorticoid-induced skin atrophy, and maintenance of the epidermis and subcutaneous adipose. In addition, our findings form the foundation for the development of safer topical glucocorticoid treatment regimens by combining this therapy with REDD1 inhibitors. Seven wk old B6D2 females in the telogen stage of the hair cycle were shaved, and treated 3 days later. Glucocorticoid FA was applied topically (2 ug/animal) in 200 ul acetone to the back skin once or up to four applications every third day as described previously (Chebotaev et al., 2007b). Control animals were treated with acetone only. Skin was harvested 24 h after last FA application or 4-8 h after single FA application as indicated in Figure legends. To evaluate the anti-inflammatory effect of glucocorticoid FA in REDD1 KO and w.t. B6x129 mice, we used ear edema test (Schoepe et al., 2010; Schäcke et al., 2004; Park et al., 2006). Seven wk old female animals were pretreated with FA or vehicle applied to the back of the ear lobe one h before application of nonspecific contact irritant croton oil . Mice were sacrificed and ears were harvested nine hours after CO application, the time point at which we observed maximum ear edema in F1 C57Bl x 129 animals (data not shown). Four mm ear punch biopsies were immediately weighted to assess ear edema. Animals were injected i.p. with bromodeoxyuridine 1 hr before skin was harvested. Total RNA from murine epidermis and whole human skin was isolated with RiboPure kit (Ambion). Total RNA from murine s.c. adipose was isolated with RNeasy Lipid Tissue Kit (Qiagen). The RNA samples were treated with TURBOTM DNase (Ambion). Purity of RNA samples isolated from epidermis and s.c. fat was confirmed by the expression of adipose and keratinocyte cell markers.

Dataset Information

Transcription profiling by high throughput of Blumeria gramis during infection of Barley

Publications

Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in extreme parasitism.

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