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ScRNA-seq analysis of lung neutrophils from mice, 24 hours after intranasal exposure to vehicle, 100 ng LPS and 10 µg LPS

ABSTRACT: Mice intranasally exposed to a low dose of LPS (i.e., 100 ng) are prone to develop features of allergic asthma upon subsequent exposure to house dust mites (HDM) allergens, while mice exposed to vehicle or 100 µg LPS do not develop such features. In order to understand the mechanisms that promote allergic asthma, we sought to characterize the lung neutrophils, which are massively recruited after LPS exposure, by single cell RNA-Seq.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Thomas Marichal

PROVIDER: E-MTAB-6902 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Locally instructed CXCR4<sup>hi</sup> neutrophils trigger environment-driven allergic asthma through the release of neutrophil extracellular traps.

Radermecker Coraline C Sabatel Catherine C Vanwinge Céline C Ruscitti Cecilia C Maréchal Pauline P Perin Fabienne F Schyns Joey J Rocks Natacha N Toussaint Marie M Cataldo Didier D Johnston Sebastian L SL Bureau Fabrice F Marichal Thomas T

Nature immunology 20191007 11

Low exposure to microbial products, respiratory viral infections and air pollution are major risk factors for allergic asthma, yet the mechanistic links between such conditions and host susceptibility to type 2 allergic disorders remain unclear. Through the use of single-cell RNA sequencing, we characterized lung neutrophils in mice exposed to a pro-allergic low dose of lipopolysaccharide (LPS) or a protective high dose of LPS before exposure to house dust mites. Unlike exposure to a high dose o ...[more]

PMID: 31591573

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