Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq of Mercurialis annua control and selected lines after four generations of selection for increased male allocation in females

ABSTRACT: A selection experiment has been set that selects females Mercurialis annua for increased allocation in male functions by depriving selected populations of all males. This resulted in a drastic phenotypic shift in sex allocation in females of the selected lines. The present RNA sequencing of control and selected lines after four generations of selection permits us to track the expression levels of previously identified male- and female-biased genes in females of the selected lines.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mercurialis annua

SUBMITTER: Guillaume Cossard

PROVIDER: E-MTAB-6905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Whole-body transcriptomics of Nasonia vitripennis females as they respond to sex allocation cues

Project description:Linking the evolution of the phenotype to the underlying genotype is a key aim of evolutionary genetics and is crucial to our understanding of how natural selection shapes a trait. Here we consider the genetic basis of sex allocation behaviour in the parasitoid wasp Nasonia vitripennis using a transcriptomics approach. Females allocate offspring sex in line with Local Mate Competition (LMC) theory. Female-biased sex ratios are produced when one or few females lay eggs on a patch. As the number of females contributing offspring to a patch increases, less female-biased sex ratios are favoured. We contrasted the transcriptomic responses of females as they oviposit under conditions known to influence sex allocation: foundress number (a social cue) and the state of the host (parasitised or not). We found, that when females encounter other females on a patch, or assess host quality with their ovipositors, the resulting changes in sex allocation is not associated with significant changes in whole-body gene expression. We also found that the gene expression changes produced by females, as they facultatively allocate sex in response to a host cue and a social cue, are very closely correlated. We expanded the list of candidate genes associated with oviposition behaviour in Nasonia, some of which may be involved in fundamental processes underlying the ability to facultatively allocate sex, including sperm storage and utilisation. 2 x 3 factorial design. Females were placed into 1 of 2 "foundress number" groups: 1) alone or 2) in the presence of 9 other females (co-foundresses). Females were further subdivided into host treatment groups: i) given no host, ii) given a fresh host and iii) given a pre-parasitised host. This gives a total of 6 possible treatment combinations. For each of these 6 groups, 7 pools of 10 females were sequenced giving 42 libraries altogether.

2015-11-11 | E-GEOD-74241 | biostudies-arrayexpress

RNAseq of Mercurialis annua control and selected lines after four generations of selection for increased male allocation in females

Project description:RNAseq of Mercurialis annua control and selected lines after four generations of selection for increased male allocation in females

| PRJEB27300 | ENA

Whole-body transcriptomics of Nasonia vitripennis females as they respond to sex allocation cues

2015-11-11 | GSE74241 | GEO

Chromatin plasticity contributes to neuronal selection during memory allocation

Project description:Memory allocation refers to the process by which neurons are recruited into the encoding ensemble to store learned information. This recruitment is regulated by neuronal selection based on differences in intrinsic excitability (IE) and the expression of the transcription factor CREB. However, whether other forms of plasticity influence memory allocation remains unknown. Here, we found that chromatin compaction and histone acetylation in the mouse lateral amygdala display a high degree of heterogeneity, a prerequisite for neuronal selection. Consequently, when we increased histone acetylation by overexpressing histone acetyl transferases (HATs), neurons with elevated histone acetylation were preferentially recruited into the encoding ensemble and memory retention was enhanced, while optogenetic silencing of the epigenetically altered neurons prevented memory expression. Mechanistically, using patch-clamp recordings and single-nucleus multi-ome sequencing, we observed that HAT overexpression increased IE and epitranscriptomic changes favoring synaptic plasticity. Lastly, by merging FRET-based epigenetic beacons with calcium indicators to simultaneously record histone acetylation and neuronal dynamics in real time, we found that epigenetic heterogeneity underlies IE in cell-autonomous manner. These results identify chromatin plasticity as a key factor catalyzing memory allocation.

2024-07-26 | GSE220696 | GEO

Chromatin plasticity contributes to neuronal selection during memory allocation

Project description:Chromatin plasticity contributes to neuronal selection during memory allocation

| PRJNA911184 | ENA

Transcription profiling of Anopheles gambiae female bodies from virgin mosquitoes and 2, 6 and 24 hours after mating

Project description:Changes in gene expression in whole Anopheles gambiae female bodies between virgin mosquitoes and females samples at 2h, 6h, and 24h after mating.

2009-01-01 | E-MEXP-1868 | biostudies-arrayexpress

The sexually antagonistic genes of Drosophila melanogaster

Project description:Differences in the selective pressures experienced by males and females are believed to be ubiquitous in dioecious organisms and are expected to result in the evolution of sexually antagonistic alleles, thereby driving the evolution of sexual dimorphism. Negative genetic correlation for fitness between the sexes has been documented, however, the identity, number and location of loci causing this relationship are unknown. Here we show that a large proportion of Drosophila melanogaster transcripts are associated with the interaction between genomic haplotype and gender and that at least 8% of loci in the fly genome are currently evolving under sexually antagonistic selection. We measured gene expression of adult males and females of Drosophila melanogaster from 15 hemiclone lines, showing either high-male/low-female fitness, high-female/lowmale fitness or average fitness in both sexes. Data from four replicates for each sex/line are presented, giving a total of 120 arrays.

2010-02-10 | E-GEOD-17013 | biostudies-arrayexpress

Post-mating expression of female Drosophila melanogaster genomes experimentally evolved under different post-copulatory sexual selection regimes

Project description:By combining an experimental evolution approach with genomic techniques, we investigated the effects of seminal fluid on female gene expression. In our study, we experimentally manipulated the mating system in replicate populations of D. melanogaster, by removing post-copulatory sexual selection, with the aim of testing differences in short term post-mating reaction of females evolved under different mating strategies. We show that monogamous females suffer decreased fecundity, regardless of the type of male they were mated with, and that their post-mating gene expression profiles differ significantly from promiscuous females, involving 1141 transcripts (9% of the genes tested). These transcripts are active in several tissues, mainly ovaries, neural tissues, midgut and spermathecae, and are involved in metabolic processes, reproduction and signaling pathways. Our results provide a list of candidate genes responsible for the decrease in female fecundity in the absence of post-copulatory sexual selection, and demonstrate how the female post-mating response can evolve under different mating systems over relatively short time frames. From an LHM base population, we created 8 replicate populations and maintained them under experimental evolution: 4 populations were allowed to mate only once every generation (monogamy), and the other 4 were kept under the standard mating protocol (promiscuous). After 46 generations, we crossed males and females within the same population and with individuals of the opposite treatment. Mated female flies were frozen 6 h after mating and RNA extracted. Two biological replicates per cross per population (2x2x8=32 samples).

2014-01-01 | E-GEOD-48385 | biostudies-arrayexpress

Differential gene expression is not required for facultative sex allocation: a transcriptome analysis of brain tissue in the parasitoid wasp Nasonia vitripennis.

Project description:Female Nasonia facultatively vary their offspring sex ratio in line with Hamilton’s theory of Local Mate Competition (LMC). A single female or “foundress” laying eggs on a patch will lay just enough sons to fertilise her daughters. As the number of “foundresses” laying eggs on a patch increases (and LMC declines) females produce increasingly male-biased sex ratios. Phenotypic studies have revealed the cues females use to estimate the level of LMC their sons will experience but our understanding of the genetics underlying sex allocation is limited. Here, we exposed females to three foundress number conditions, i.e. three LMC conditions, and allowed them to oviposit. mRNA was extracted from only the heads of these females to target the brain tissue. The subsequent RNA-seq experiment confirmed that differential gene expression is not associated with the response to sex allocation cues and that we must instead turn to the underlying neuroscience to reveal the underpinnings of this impressive behavioural plasticity.

2018-01-11 | GSE105796 | GEO

Transcription profiling of Drosophila melanogaster selected for aggressive behavior vs non aggressive control

Project description:We generated 4 separate subpopulations of selected flies from 1 starting population of a mixture of Canton-S flies. Two of the populations (AggrI and AggrII) were selected for increased aggression by picking males from a population cage that engaged in the most intense fighting (known as escalation behavior). Thirty males were selected every generation (from a total of 120 males per cage) per line and mated with random virgin females from that same generation. Two control populations (NeutrI and NeutrII) were selected by picking random males from the population cage after 15-30 aggressive males were removed. After 21 generations of selection males from the Aggr lines were dramatically more aggressive than males from the Neutr lines. The Neutr lines did not significantly differ from the starting population although a trend of decreased aggression was visible. Experiment Overall Design: Four populations were analyzed. Two were selected for increased aggression and two were reference groups, selected for a decrease but with no significant effect.

2007-11-01 | E-GEOD-5335 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data