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Time series experiment on L4 stage of Caenorhabditis elegans N2

ABSTRACT: This experiment investigates the transcriptional effect of age in the N2 strain of Caenorhabditis elegans during the L4 stage. The goal is to identify genes that show dynamic gene-expression patterns over time. We grew N2 populations over the course of 44 to 58 hours at 20 degrees Celsius, independent populations were harvested in hourly intervals. Thereafter RNA was isolated, labelled and hybridized on microarray. The gene expression profiles were analyzed for dynamic expression patterns using principal component analysis and k-means clustering.

INSTRUMENT(S): agilent high resolution C scanner

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Mark Sterken

PROVIDER: E-MTAB-7019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A rapid and massive gene expression shift marking adolescent transition in C. elegans.

Snoek L Basten LB Sterken Mark G MG Volkers Rita J M RJ Klatter Mirre M Bosman Kobus J KJ Bevers Roel P J RP Riksen Joost A G JA Smant Geert G Cossins Andrew R AR Kammenga Jan E JE

Scientific reports 20140128

Organismal development is the most dynamic period of the life cycle, yet we have only a rough understanding of the dynamics of gene expression during adolescent transition. Here we show that adolescence in Caenorhabditis elegans is characterized by a spectacular expression shift of conserved and highly polymorphic genes. Using a high resolution time series we found that in adolescent worms over 10,000 genes changed their expression. These genes were clustered according to their expression patter ...[more]

PMID: 24468752

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