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Gene expression effects in Caenorhabditis elegans strains of feeding on Erwinia rhapontici or Escherichia coli

ABSTRACT: Diet can have a strong impact on development, to test this effect the strains N2, CB4856, three strains from Orsay (JU1581, JU1944, and JU1949), and three strains from Santeuil (JU1921, JU1930, and JU1932) were grown on two different food-sources. These sources were the standard lab food, Escherichia coli OP50, and the Gram-negative plant pathogen Erwinia rhapontici. The effect of these bacteria on development could also be measured by the start of reproduction, which was delayed while feeding on E. coli. To measure the transcriptional effects, age-synchronized strains were grown for 48 hours at 20 degrees Celsius. The impact of food-source on gene expression was determined by a transcriptional ruler based on the strain N2 (E-MTAB-7019).

INSTRUMENT(S): Maxwell 16 Instrument, Agilent High resolution C scanner

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Mark Sterken

PROVIDER: E-MTAB-8129 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A rapid and massive gene expression shift marking adolescent transition in C. elegans.

Snoek L Basten LB Sterken Mark G MG Volkers Rita J M RJ Klatter Mirre M Bosman Kobus J KJ Bevers Roel P J RP Riksen Joost A G JA Smant Geert G Cossins Andrew R AR Kammenga Jan E JE

Scientific reports 20140128

Organismal development is the most dynamic period of the life cycle, yet we have only a rough understanding of the dynamics of gene expression during adolescent transition. Here we show that adolescence in Caenorhabditis elegans is characterized by a spectacular expression shift of conserved and highly polymorphic genes. Using a high resolution time series we found that in adolescent worms over 10,000 genes changed their expression. These genes were clustered according to their expression patter ...[more]

PMID: 24468752

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