Full transcriptome analysis of MCF-7 cells treated with screening hit compounds versus control
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ABSTRACT: This experiment was performed as part of a hit screening project. Transcriptome analysis was performed to determine whether the screened hits induced a gene expression pattern consistent with proteasome inhibition. MCF-7 cells were exposed to 2x[IC50] of hit compounds for six hours. RNA was processed according to manufacturer's protocol for preparation of HuGene 2.0ST chips to acquire biotinylated sense-strand DNA. Biotinylated DNA was hybridized to HuGene 2.0ST chips at 37°C for 16 hours in a GeneChip® Hybridization Oven 645, washed and stained using the GeneChip® Fluidics Station 450 using the FS450_0002 protocol and scanned with a GeneChip® Scanner 3000 7G.
ORGANISM(S): Homo sapiens
SUBMITTER: Arjan Mofers
PROVIDER: E-MTAB-7024 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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