Unknown,Transcriptomics,Genomics,Proteomics

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Illumina microarray analysis of MCF7 and SHSY5Y cells exposed to bilirubin


ABSTRACT: To gain an insight into the molecular mechanisms by which bilirubin induces toxicity, we exposed human MCF7 and SHSY5Y cell lines to 50 uM bilirubin (which equates to 6.39 uM of unconjugated bilirubin) for 4 hours. The cells were then washed with PBS and the RNA extracted using Tri-Reagent/1-bromo-3-chloropropane phase separation. The data was extracted using GenomeStudio and normalized/analyzed using ArrayTrack.

ORGANISM(S): Homo sapiens

SUBMITTER: Joshua Smalley 

PROVIDER: E-MTAB-7096 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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