Microarray for LPS-stimulated B cells compared to unstimulated B cells
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ABSTRACT: Both multiple myeloma (MM) and systemic lupus erythematosus (SLE) are characterized with abnormal production of plasma cells. To explore the novel therapeutic target to plasma cells, we determined the gene expression profile in plasma cells by affymetrix microarrays. LPS could effectively in vitro induce the production of plasma cells. The splenocytes were separated from three C57BL/6 mice per group. B cells were sorted by B220 microbeads, and stimulated for 3 days in vitro by 10 ug/ml LPS. The transcripts in LPS-stimulated B cells were determined by Affymetrix Microarrays.
INSTRUMENT(S): Agilent 2100 Bioanalyzer, Affymetrix mouse gene 1.0 ST arrays, GeneChip Instrument System
ORGANISM(S): Mus musculus
SUBMITTER: Renxi Wang
PROVIDER: E-MTAB-7109 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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