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RNA-seq of mouse myeloma cell line SP 2/0 overexpressed with Gm614 against vector control

ABSTRACT: We found that a novel gene Gm614 mRNA significantly changed in plasma cells. Because of plasmablast-like, Mus musculus myeloma SP 2/0 cell line was selected to test the effect of Gm614 overexpression on plasmablast/plasma cells. Gm614 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector LV201 (Fugene Corp., Guangzhou, China) to generate Gm614 protein. Gm614-expressing LV201 was then transfected into SP 2/0 cells, and stable transfectants were identified by drug selection (Puromycin, Sigma, 10 μg/ml). To identify the effect of Gm614 overexpression on gene expression, we determined mRNA profiles in Gm614-overexpressed and vector-transduced SP 2/0 cells by RNA-seq. RNA-seq was done with an Illumina HiSeq 2500 instrument at GENEWIZ, Suzhou, China.

INSTRUMENT(S): Agilent 2100 Bioanalyzer, Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Renxi Wang

PROVIDER: E-MTAB-7135 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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