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RNA-seq expression profiling of humain MAIT cells in blood and liver as compared to mainstream TCD4+ and TCD8+ cells.

ABSTRACT: At variance with what is observed in mice, no distinct MAIT1 or MAIT17 subsets exist in human blood, as all MAIT cells express a variety of transcription factors such as Rorgt, Tbet, Eomes and Helios. However, they are also found in tissues in which they have specific effector functions. To determine these tissue programs, we analyzed the transcription pattern of MAIT cells as compared to mainstream memory (CD45RA-CD27+) CD4+ and CD8+ T cells from human blood and liver. The paired samples of blood and liver cells were obtained from patients operated for metastatic uveal melanoma (liver samples from a “healthy” liver fragment), and from the blood of healthy controls.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Olivier LANTZ

PROVIDER: E-MTAB-7143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A common transcriptomic program acquired in the thymus defines tissue residency of MAIT and NKT subsets.

Salou Marion M Legoux François F Gilet Jules J Darbois Aurélie A du Halgouet Anastasia A Alonso Ruby R Richer Wilfrid W Goubet Anne-Gaëlle AG Daviaud Céline C Menger Laurie L Procopio Emanuele E Premel Virginie V Lantz Olivier O

The Journal of experimental medicine 20181205 1

Mucosal-associated invariant T (MAIT) cells are abundant T cells with unique specificity for microbial metabolites. MAIT conservation along evolution indicates important functions, but their low frequency in mice has hampered their detailed characterization. Here, we performed the first transcriptomic analysis of murine MAIT cells. MAIT1 (RORγt<sup>neg</sup>) and MAIT17 (RORγt<sup>+</sup>) subsets were markedly distinct from mainstream T cells, but quasi-identical to NKT1 and NKT17 subsets. The ...[more]

PMID: 30518599

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Transcription profiling of human CD4+T-cells, Th1/Th2 polarized time-series and primary memory subsets

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