Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

MRNA expression of Atopic Dermatitis skin

ABSTRACT: mRNA array analysis was carried out using total RNA of skin biopsies from lesional and non-lesional skin of three atopic dermatitits patients and four healthy individuals.

ORGANISM(S): Homo sapiens

DISEASE(S): normal

SUBMITTER: Ana Rebane

PROVIDER: E-MTAB-729 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mechanisms of IFN-γ-induced apoptosis of human skin keratinocytes in patients with atopic dermatitis.

Rebane Ana A Zimmermann Maya M Aab Alar A Baurecht Hansjörg H Koreck Andrea A Karelson Maire M Abram Kristi K Metsalu Tauno T Pihlap Maire M Meyer Norbert N Fölster-Holst Regina R Nagy Nikoletta N Kemeny Lajos L Kingo Külli K Vilo Jaak J Illig Thomas T Akdis Mübeccel M Franke Andre A Novak Natalija N Weidinger Stephan S Akdis Cezmi A CA

The Journal of allergy and clinical immunology 20120324 5

<h4>Background</h4>Enhanced apoptosis of keratinocytes is the main cause of eczema and spongiosis in patients with the common inflammatory skin disease atopic dermatitis (AD).<h4>Objective</h4>The aim of the study was to investigate molecular mechanisms of AD-related apoptosis of keratinocytes.<h4>Methods</h4>Primary keratinocytes isolated from patients with AD and healthy donors were used to study apoptosis by using annexin V/7-aminoactinomycin D staining. Illumina mRNA Expression BeadChips, qu ...[more]

PMID: 22445417

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Project description:Background: Plaque psoriasis is a chronic autoimmune disorder characterized by the development of red scaly plaques. To date psoriasis lesional skin transcriptome has been extensively studied, whereas only few proteomic studies of psoriatic skin are available. Aim: The aim of this study was to compare protein expression patterns of lesional and normally looking skin of psoriasis patients with skin of the healthy volunteers, reveal differentially expressed proteins and identify changes in cell metabolism caused by the disease. Methods: Skin samples of normally looking and lesional skin donated by psoriasis patients (n = 5) and samples of healthy skin donated by volunteers (n = 5) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After protein identification and data processing, the set of differentially expressed proteins was subjected to protein ontology analysis to characterize changes in biological processes, cell components and molecular functions in the patients' skin compared to skin of the healthy volunteers. Results: The performed analysis identified 405 and 59 differentially expressed proteins in lesional and normally looking psoriatic skin compared to healthy control. We discovered decreased expression of KNG1, APOE, HRG, THBS1 and PLG in normally looking skin of the patients. Presumably, these changes were needed to protect the epidermis from spontaneous activation of kallikrein-kinin system and delay the following development of inflammatory response. In lesional skin, we identified several large groups of proteins with coordinated expression. Mainly, these proteins were involved in different aspects of protein and RNA metabolism, namely ATP synthesis and consumption; intracellular trafficking of membrane-bound vesicles, pre-RNA processing, translation, chaperoning and degradation in proteasomes/immunoproteasomes. Conclusion: Our findings explain the molecular basis of metabolic changes caused by disease in skin lesions, such as faster cell turnover and higher metabolic rate. They also indicate on downregulation of kallikrein-kinin system in normally looking skin of the patients that would be needed to delay exacerbation of the disease.

Dataset Information

MRNA expression of Atopic Dermatitis skin

Publications

Mechanisms of IFN-γ-induced apoptosis of human skin keratinocytes in patients with atopic dermatitis.

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