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Barley husk adhesion correlates with changes in gene expression associated with plant cuticle biosynthesis and caryopsis cuticle composition

ABSTRACT: Caryopses of barley grains become firmly adhered to the glumes of the husk during grain development through a cuticular cementing layer on the caryopsis surface. The quality of this attachment varies among cultivars, with poor quality adhesion causing “skinning”, an economically significant grain quality defect for the malting industry. Malting cultivars encompassing a range of husk adhesion qualities were grown under a misting treatment known to induce skinning. Changes in gene expression during adhesion development were examined with a custom barley microarray. The abundance of transcripts involved early in cuticular lipid biosynthesis, including acetyl-CoA carboxylase, and all four members of the fatty acid elongase complex of enzymes was significantly higher early in caryopsis development than later. Members of the subsequent cuticular lipid biosynthetic pathways were also higher early in development including the decarbonylation and reductive pathways, and sterol biosynthesis.

INSTRUMENT(S): Agilent G2505B scanner

ORGANISM(S): Hordeum vulgare subsp. vulgare

SUBMITTER: Pete Hedley

PROVIDER: E-MTAB-7429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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