Project description:The hypothesis that the onset and development of colorectal cancer result from the alteration of the close cooperation of mRNA-miRNA has gained increasing popularity. We performed a multifaceted enrichment analysis of hypernetworks, taking advantage of the simultaneous evaluation of transcriptome and miRNAome, to search deregulated micro-societies of mRNAs and miRNAs involved in signaling pathways that become critical when altered in colorectal carcinogenesis.
Project description:Comparison of the miRNAs profile of 8 WHO grade II gliomas and 24 higher grade tumors (2 WHO grade III and 22 glioblastomas) in order to identify the molecular determinants of progression.
Project description:we analyze expression of miRNAs in a cohort of male and female patients with familial breast cancer (BRCA1/2-related and BRCAX) and in a subset of sporadic breast cancer
Project description:This study aimed to uncover new circulating microRNAs (miRNAs) that would distinguish patients with pancreatic cancer (PanC) from healthy subjects (HS), and predict patients’ clinical phenotypes and outcomes.
Project description:NIH 3T3 cells (mouse fibroblasts) were engineered such that the dominant negative ATPase-defective BRG-1 is expressed via the tet-off inducible expression system to inactivate the SWI-SNF chromatin remodeling complexes (Mol Cell Biol 20, 2839). These cell lines have been used for several studies to demonstrate the in vivo functions of the SWI-SNF complexes in transcription, differentiation and cell cycle control. We used one of those cell line designated as B05-1. B05-1 cells were cultured in the medium with tetracycline for routine maintenance, and cultured in the medium without tetracycline for four days to maximally induce the expression of the dominant negative BRG-1, which we designated as B05-1(+tet) and B05-1(-tet) cells, respectively. The aim of our microarray experiments was to determine the genes whose expression levels are changed by inactivation of the SWI-SNF complexes by analyzing the genes differentially expressed between B05-1(+tet) and B05-1(-tet) cells. It should be noted that, since the comparison was made between B05-1 cells cultured with and without tetracycline for four days, the genes differentially expressed between these two cells do not only represent the genes that are directly regulated by the SWI-SNF complexes but also include the genes that are secondarily affected by SWI-SNF inactivation as well as the genes whose expression is changed by tetracycline effect per se.
Project description:miRNAs of at least 2 times expression level higher in seeds were all from the miR319, miR171 and miR166 family. In leaves, most up-regulated miRNAs included miR399a/c/d/e, miR408, miR159c/d, miR156k/j, miR160a/b/c/d/e and miR164a/b/c/d We intend to identify the up-regulated miRNAs in maize seed and screen for key miRNAs involved in maize seed development
Project description:Synovial sarcomas account for approximately 10% of all soft-tissue tumors and occur most frequently in young adults. A specific translocation in this sarcoma induces fusion of the SYT gene on chromosome 18 to the SSX genes on chromosome X, leading to proliferation of the tumor cells. The need for non-invasive biomarkers indicating recurrence and activity of this disease has sparked research into short non-coding RNA known as microRNA (miRNA). Patients and Methods: Serum samples of patients with active synovial sarcoma, healthy donors and leiomyosarcoma patients were collected. Cell-free serum was obtained by differential centrifugation steps and analyzed using an Affymetrix GeneChip miRNA Array v. 4.0. qRT-PCR was carried out to confirm a panel of miRNAs which where differentially expressed in the miRNA array.