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Single cell RNA seq of influenza tetramer specific CD8+ T cells from baseline, acute and long term memory timepoints

ABSTRACT: The aim of the experiment was to assess the differentiation and activation status of influenza-specific CD8+ T cells (A2/PB1 413 and A2/BHA 543) from an individual naturally infected with influenza B (PCR-confirmed). Single tetramer+ CD8+ T cells from tetramer-enriched PBMCs were sorted from baseline (~3 months before infection), at 14 days after infections, at 3 months after infection and at 1.5 years after infection. Libraries were prepared using a modified Smart-seq2 protocol as detailed in the methods section of the manuscript.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Fabio Luciani 

PROVIDER: E-MTAB-7606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Human CD8<sup>+</sup> T cell cross-reactivity across influenza A, B and C viruses.

Koutsakos Marios M   Illing Patricia T PT   Nguyen Thi H O THO   Mifsud Nicole A NA   Crawford Jeremy Chase JC   Rizzetto Simone S   Eltahla Auda A AA   Clemens E Bridie EB   Sant Sneha S   Chua Brendon Y BY   Wong Chinn Yi CY   Allen E Kaitlynn EK   Teng Don D   Dash Pradyot P   Boyd David F DF   Grzelak Ludivine L   Zeng Weiguang W   Hurt Aeron C AC   Barr Ian I   Rockman Steve S   Jackson David C DC   Kotsimbos Tom C TC   Cheng Allen C AC   Richards Michael M   Westall Glen P GP   Loudovaris Thomas T   Mannering Stuart I SI   Elliott Michael M   Tangye Stuart G SG   Wakim Linda M LM   Rossjohn Jamie J   Vijaykrishna Dhanasekaran D   Luciani Fabio F   Thomas Paul G PG   Gras Stephanie S   Purcell Anthony W AW   Kedzierska Katherine K  

Nature immunology 20190218 5

Influenza A, B and C viruses (IAV, IBV and ICV, respectively) circulate globally and infect humans, with IAV and IBV causing the most severe disease. CD8<sup>+</sup> T cells confer cross-protection against IAV strains, however the responses of CD8<sup>+</sup> T cells to IBV and ICV are understudied. We investigated the breadth of CD8<sup>+</sup> T cell cross-recognition and provide evidence of CD8<sup>+</sup> T cell cross-reactivity across IAV, IBV and ICV. We identified immunodominant CD8<sup>+  ...[more]

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