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Human breast cancer cell line temporal response to Hepatocyte growth factor / scatter factor (HGF/SF)

ABSTRACT: To study the gene expression kinetics following activation of Met, one normal human breast epithelium and six human breast cancer cell lines with variable Met expression and activity, were treated with HGF/SF for 0, 10, 30min and 24 hours and subjected to cDNA array analysis

ORGANISM(S): Homo Sapiens

DISEASE(S): normal

SUBMITTER: Gideon Stein

PROVIDER: E-MTAB-762 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Met kinetic signature derived from the response to HGF/SF in a cellular model predicts breast cancer patient survival.

Stein Gideon Y GY Yosef Nir N Reichman Hadar H Horev Judith J Laser-Azogui Adi A Berens Angelique A Resau James J Ruppin Eytan E Sharan Roded R Tsarfaty Ilan I

PloS one 20120925 9

To determine the signaling pathways leading from Met activation to metastasis and poor prognosis, we measured the kinetic gene alterations in breast cancer cell lines in response to HGF/SF. Using a network inference tool we analyzed the putative protein-protein interaction pathways leading from Met to these genes and studied their specificity to Met and prognostic potential. We identified a Met kinetic signature consisting of 131 genes. The signature correlates with Met activation and with respo ...[more]

PMID: 23049908

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