Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

U1 RAP-RNA-Seq analysis of A2Lox mouse embryonic stem cells treated with a control or Srrt-specific siRNA


ABSTRACT: The experiment was carried out to examine the effect of Srrt knockdown on the U1 snRNP/pre-mRNA interaction pattern. A2Lox mouse embryonic stem cells were transfected with either an Srrt-specific or a non-targeting siRNA. 48 hours post transfection the cells were cross-linked using 2% formaldehyde and lysed. RNA was partially fragmented by sonication, hybridized with U1 snRNA-specific biotinilated probes and pulled down. U1-associated RNA sequences were then purified and RNA-seq libraries were generated using a NEBNext® rRNA Depletion Kit and NEBNext® Ultra8482 II Directional RNA Library Preparation kit. Paired-end sequencing was performed using a HiSeq4000 75bp platform.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Yaroslav Kainov 

PROVIDER: E-MTAB-7635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2022-09-30 | E-MTAB-12077 | biostudies-arrayexpress
2024-11-01 | E-MTAB-13566 | biostudies-arrayexpress
2023-04-07 | E-MTAB-12800 | biostudies-arrayexpress
2024-08-31 | E-MTAB-14308 | biostudies-arrayexpress
2024-08-31 | E-MTAB-14309 | biostudies-arrayexpress
2020-05-01 | E-MTAB-7670 | biostudies-arrayexpress
2021-11-05 | E-MTAB-10365 | biostudies-arrayexpress
2023-01-08 | E-MTAB-11544 | biostudies-arrayexpress
2022-06-24 | E-MTAB-10848 | biostudies-arrayexpress
2023-08-03 | E-MTAB-12180 | biostudies-arrayexpress