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Transcriptome analysis of JAK2wt and JAK2V617F+ iPSCs and iPSC-derived CD34+ progenitor-enriched cultures, either inflammatory cytokines treated or untreated.

ABSTRACT: PV-patient specific and control healthy individual-derived iPSCs were upon differentiation for 9 days into CD34+ hematopoietic progenitors treated with IFNγ, TNFα and TGFβ1 or untreated and used for transcriptome analysis. This dataset shows presence of cell-autonomous and strong non-cell autonomous JAK2V617F-dependet inflammatory footprint at the transcriptome level.

ORGANISM(S): Homo sapiens

SUBMITTER: Jiri Bartek

PROVIDER: E-MTAB-7693 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Addiction to DUSP1 protects JAK2V617F-driven polycythemia vera progenitors against inflammatory stress and DNA damage, allowing chronic proliferation.

Stetka J J Vyhlidalova P P Lanikova L L Koralkova P P Gursky J J Hlusi A A Flodr P P Hubackova S S Bartek J J Hodny Z Z Divoky V V

Oncogene 20190409 28

Inflammatory and oncogenic signaling converge in disease evolution of BCR-ABL-negative myeloproliferative neoplasms, clonal hematopoietic stem cell disorders characterized by gain-of-function mutation in JAK2 kinase (JAK2V617F), with highest prevalence in patients with polycythemia vera (PV). Despite the high risk, DNA-damaging inflammatory microenvironment, PV progenitors tend to preserve their genomic stability over decades until their progression to post-PV myelofibrosis/acute myeloid leukemi ...[more]

PMID: 30967632

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