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Unknown,Transcriptomics,Genomics,Proteomics

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Expression profiling in HGF (Hereditary Gingival Fibromatosis) patients

ABSTRACT: To identify dysregulated genes in HGF (Hereditary Gingival Fibromatosis) patients, expression profiling was performed using Affymetrix Human Genome U133 plus 2.0 arrays. Total RNA was extracted from gingival tissue specimens excised from two non-syndromic HGF patients, siblings in one family, and three normal controls. Quantity and integrity of the total RNA was measured and assessed by Nanodrop spectrophotometer (Thermo Fisher Scientific) and agarose gel electrophoresis. In brief, cDNA preparation, cRNA labeling, hybridization to Affymetrix Human Genome U133 plus 2.0 arrays, and chip scanning were performed in accordance with the manufacturer’s protocol (Affymetrix). *.CEL data from the raw chip scans were imported into the Partek GS 6.5 and normalized by RMA normalization algorithms. Comparisons between the HGF and control group were performed with one-way ANOVA (p≤0.05).

ORGANISM(S): Homo sapiens

SUBMITTER: KAI YANG

PROVIDER: E-MTAB-7801 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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