Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq of follicles from Zrsr2 WT and Zrsr2 mutant females.

ABSTRACT: Zrsr2 is an essential minor splicing factor. To investigate the effects of minor splicing during oogenesis, Zrsr2 mutant female mice were produced. Oogenesis was blocked at the secondary follicle stage, so follicles were recovered, and total RNA was extracted and sequenced to determine the genetic basis of this phenotype.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Isabel Gómez Redondo

PROVIDER: E-MTAB-7806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of Zrsr1-2WT and Zrsr1-2 mutant embryos

Project description:Zrsr1 and Zrsr2 are two paralogous genes, whose products play a key role in minor splicing. To investigate the effects of minor splicing during early embryo development, embryos from Zrsr2 mutant females and Zrsr1 mutant males were produced, showing an impaired development during zygotic gene activation at the 2-cell stage. RNA-seq of these embryos was performed to check the complementation of these two genes during this stage.

2020-06-15 | E-MTAB-9102 | biostudies-arrayexpress

Microarray profiling of UPF2-deficient mouse GV oocytes.

Project description:To study the role of UPF2 during oogenesis, Upf2 was conditionally deleted in growing oocytes. Total RNA samples from UPF2-deficient GV oocytes were subjected to array profiling. The control samples for this experiment can be found in E-MTAB-5056.

2019-01-14 | E-MTAB-7064 | biostudies-arrayexpress

RNA-seq of hypothalamus from Zrsr1 WT and Zrsr1 mutant mice.

Project description:Splicing factor ZRSR1 is known to have a role in spermatogenesis and fertility, leading its mutation to several reproductive defects in male. To investigate if Zrsr1 gene is involved in male reproductive control by the hypothalamus, we performed RNA-seq to determine the differences in gene expression, usage of isoforms and alternative splicing between the hypothalamus of WT mice and from Zrsr1mu mice.

2019-07-19 | E-MTAB-8012 | biostudies-arrayexpress

Impaired spermatogenesis, muscle and erythrocyte function in U12 intron splicing-defective Zrsr1 mutant mice

Project description:We analysed the gene expression in testis of Zrsr1 mutant mice. We generated mutant mice containing truncating mutations within the RNA-recognition motif (RRM) in Zrsr1 locus. The RNAs of testes from 4 WT and 5 Zrsr1 mutant mice at day 15, immediately after the peak of expression of Zrsr1 in developing testis, were prepared and sequenced in order to identify and characterize the RNAs regulated by Zrsr1 in developing testis.

2018-04-03 | E-MTAB-4872 | biostudies-arrayexpress

The faint sausage gene encodes a subunit of the spliceosome-activating Prp19 complex and is required for tracheal branching morphogenesis in Drosophila

Project description:Morphogenesis requires the dynamic regulation of gene expression, including transcription, mRNA maturation and translation. Dysfunction of general components of the splicing machinery can cause surprisingly specific phenotypes, but the basis for these cell-type specific effects is not clear. Here we show that the faint sausage (fas) locus, implicated in epithelial morphogenesis and previously reported to encode a secreted immunoglobulin domain protein, in fact encodes a subunit of the Prp19 complex that is essential for efficient pre-mRNA splicing. Loss of zygotic fas function impairs the efficiency of splicing, associated with widespread retention of introns in mature mRNAs and dramatic changes in gene expression. Surprisingly, despite these general effects, zygotic fas mutants show specific defects in tracheal cell migration. Zygotic fas function becomes essential during late embryogenesis when maternally supplied splicing factors decline. We propose that tracheal branching, which relies on dynamic changes in gene expression, is particularly sensitive for efficient spliceosome function. Our results provide an entry point to study functions of splicing during organogenesis and provide a better understanding of specific disease phenotypes associated with mutations in general splicing factors.

2016-11-18 | E-MTAB-5069 | biostudies-arrayexpress

Zebrafish ovarian follicle transcriptome

Project description:SAGE analysis of fully grown ovarian follicles has been carried out to supply a resource for identification of important molecules involved in vertebrate oogenesis and in early stages of embryonic development. Keywords: SAGE About 250 full-grown ovarian follicles were obtained after gentle squeezing the abdomen of five mature zebrafish females.

2006-03-17 | E-GEOD-3679 | biostudies-arrayexpress

RNA-seq of HeLA SS6 cells treated with siRNAs against Smu1, RED/IK, MFAP1

Project description:Assessment of effects on splicing efficiency of knockdowns of select splicing factors (Smu1, RED/IK, MFAP1)

2019-06-01 | E-MTAB-7522 | biostudies-arrayexpress

Human telomerase RNA processing and quality control

Project description:RNA sequencing of HeLa cells treated with siRNA against the RNA exosome components hRRP40, hRRP6, hDIS3, and hRRP6/hDIS3 or the splicing inhibitors Isoginkgetin and spliceostatin A, respectively. Stranded, ribo-depleted RNA seq profiles of HeLa cells treated with exosome targeting siRNAs or splicing inhibitors using Illumina HiSeq. All experiments were carried out in triplicate starting with independent cell cultures

2015-11-25 | E-GEOD-72055 | biostudies-arrayexpress

Aedes aegypti mosquito eggshell proteins in response to RNAi-EOF1

Project description:Aedes aegypti mosquito ovarian follicles develop synchronously after taking a blood meal. A single layer of follicular epithelial cells surrounding the oocyte is responsible for secreting a majority of eggshell structural components between 18 and 54 hours post blood meal. We previously identified a protein called eggshell organizing factor 1 (EOF1). When we knocked down EOF1 with RNA interference in female adult mosquitoes, they laid eggs that were not properly melanized, and fragile eggs did not contain viable embryos. Here, we aimed to determine putative downstream eggshell proteins in RNAi-EOF1 female mosquitoes. Our eggshell proteomics identified 220 proteins, suggesting that mosquito extracellular eggshells are composed of a complex mixture of proteins. These proteins are involved in the formation of an intact eggshell that protects the embryonic development and larva from the environment for long periods of time.

2023-09-29 | PXD045606 | Pride

hDBR1 OVEREXPRESSED CALU6 AND 293T CELLS VS. CONTROL CELLS

Project description:RNA debranching enzyme (hDBR1) is the only known enzyme to turn over lariat intron RNA during splicing. We compared the transcriptome differences between hDBR1 overexpressed and control Calu6 and 293T cells, to see its general effect on RNA splicing.

2017-01-25 | E-MTAB-5347 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data