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Microarray of young adults of endu-2(tm4977) loss of function mutant and endu-2(tm4977);Ex[endu-2::EGFP] rescue strain grown up at 25°C

ABSTRACT: The poly-U specific endoribonuclease ENDU-2 plays an important role in maintaining germline immortality in C. elegans. The endu-2 loss-of function mutants display a mortal germline phenotype at 20°C but not at 15°C. At 25°C, the mortal germline phenotype is enhanced. In addition, an extrachromosomal endu-2::EGFP transgene rescues the mutant phenotype. In order to investigate the role of ENDU-2 on mRNA abundancy, a microarray analysis was performed via comparing young adults of endu-2(tm4977) loss of function mutant and endu-2(tm4977);Ex[endu-2::EGFP] rescue strain grown up at 25°C. As ENDU-2 affects maternal inheritance, grouped animals for comparison were derived from one grandmother (endu-2(tm4977);Ex[endu-2::EGFP]) to ensure the same maternal inheritance at the parental generation.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Wenjing Qi

PROVIDER: E-MTAB-7993 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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