Dataset Information

Lable-free LC-MSMS in HeLa on DYRK3 and Sec16A, Human cervical cancer cells

ABSTRACT: In order to identify sites of the SEC16A specifically phosphorylated by the Dual Specificity Kinase DYRK3, mCherry-SEC16A was transiently over expressed in HeLa cells together with GFP-DYRK3 or EGFP-C1 only

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Katrina Meyer

LAB HEAD: Lucas Pelkmans

PROVIDER: PXD041620 | Pride | 2023-06-23

REPOSITORIES: Pride

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Source:

			Action	DRS
	20190418_002_S195507_Sec16_DYRK3d_Phospho.raw	Raw
	20190418_004_S195502_Sec16_GFPc_Phospho.raw	Raw
	20190418_007_S195504_Sec16_DYRK3a_Phospho.raw	Raw
	20190418_008_S195506_Sec16_DYRK3c_Phospho.raw	Raw
	20190418_009_S195500_Sec16_GFPa_Phospho.raw	Raw

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Project description:The plant response may be triggered by various factors, including pathogens, non-pathogenic microbes, and natural or synthetic molecules, such as salicylic acid (SA), benzo(1,2,3)-Thiadiazole-7-Carbothioic Acid S-Methyl Ester (BTH) that are considered as plant resistance inducers. Resistance inducers mobilize the plant for the synthesis of defense compounds, but they are not directly toxic for the plant. The BTH is an analogue of SA, a molecule naturally synthesized in the plant during systemic acquired resistance (SAR). In this study, we hypothesized that challenging the plant with resistance inducer causes an increase in the level of proteins associated with defence response to external stimuli with primary metabolism and that the action of the choline derivative of the BTH (in the form of the ionic liquid) during induction of resistance in tomato plants is mediated by to some extent similar signaling pathways as it is in the case of unmodified BTH compound. Therefore, this study aimed to identify global proteome changes occurring in tomato plants exposed to resistance inducers as well as to identify common pathways associated with the plant's defense response to (+)ssRNA viruses induced upon both, viral infection and prior resistance inducer treatment. The effect of ionic liquids on changes in plant proteome is not described in the literature. The ionic character of compounds offers a great advantage of using them as the plant resistance inducer. The high chemical and thermal stability, as well as low volatility, guarantees safe application in fields. For that reason, comparing the action of the resistance inducer in the form of an ionic liquid with the core compound (BTH) will help to elucidate the mode of action of ionic liquid derivatives in plant resistance induction. To this end, two RNA-type viruses from different families were tested: tomato mosaic virus (ToMV, Virgaviridae) and potato virus Y (PVY, Potyviridae). The most affected processes presented in the results were: photosynthesis, regulation of oxidative stress, metabolism of glutathione and chitin, cell wall reorganization. The increased impact on the regulation of primary metabolism in inducer-treated plants (both for BTH and cholinium ionic liquid derivative). The response to ToMV and PVY was more intense when infected plants were pre-treated with inducers. The increased abundances of defense priming proteins with changes in cell wall organization may block virus transport from infected spots to other tissue of the host plant so that the losses caused by the disease will be lower.

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Lable-free LC-MSMS in HeLa on DYRK3 and Sec16A, Human cervical cancer cells

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