Comparison of thymic mTEC cells from MyD88fl/fl x Foxn1Cre and MyD88fl/fl mice by transcription profiling
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ABSTRACT: We compared in vitro mTECs-high from MyD88fl/fl x Foxn1Cre and MyD88fl/fl mice: Thymi were enzymaticaly digested, cells were MACS enriched for CD45- fraction, and FACS sorted using BD Influx sorter. mTECs-high were gated as EpCAM+CD11c-Ly51-MHCII+CD80+. Total RNA was isolated using RNeasy Plus Micro Kit (Qiagen). 5 samples per condition were used.
INSTRUMENT(S): NextSeq 500
ORGANISM(S): Mus musculus
SUBMITTER: Matouš Vobořil
PROVIDER: E-MTAB-8024 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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