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RNA-seq of CSMD1 enhancer CRISPRi in LUHMES cells and differentiated neurons

ABSTRACT: we performed lentiviral CRISPR interference (CRISPRi) by recruiting dCas9 fused with the KRAB domain to the CSMD1 enhancer (fam3) in the neuronal precursor cell line – Lund human mesencephalic (LUHMES). Given that the expression of CSMD1 was not detectable in LUHMES cells we differentiated these cells into neurons. Differentiated neurons with CRISPRi of CSMD1 enhancer showed significantly higher expression of CSMD1 than control.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Santosh Atanur

PROVIDER: E-MTAB-8027 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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