RNA-seq of human cell line HeLa after depletion of a lncRNA with three different loss-of-function methods

ABSTRACT: Long noncoding RNAs (lncRNAs) are a major transcriptional output of the mammalian genome, and their cellular roles are typically assayed by a variety of loss-of-function approaches. This study aims to identify the best current method to deplete nuclear lncRNAs. Small interfering RNAs (RNAi), antisense oligonucleotides (LNAs) and CRISPR interference (CRISPRi) were applied to knock down loc100289019 (a typical nuclear lncRNA, referred to as lnc289) in HeLa cells. We generated sequencing libraries after performing each step of each method, up to and including depletion of lnc289. Differential expression analyses between libraries generated before and after each step allowed us to evaluate the effect of that step on gene expression. The transcriptional effect of lncRNA depletion was then compared to the magnitude of off-target effects inherent to each method.

INSTRUMENT(S): Illumina HiSeq 2500, 2100 Bioanalyzer, BD FACSAria III cell sorter, Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Aaron Lun 

PROVIDER: E-MTAB-5308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress