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Cardiac miRNA expression changes induced by prediabetes

ABSTRACT: The aim of the experiment was the analysis of key molecular changes with an unbiased, transcriptomics-based approach in a prediabetes model. Small RNA sequencing analysis was applied to explore the expression changes resulted high-fat chow (supplemented with 40% lard) diet for 21 weeks and a single low dose of streptozotocin (20 mg/kg) treatment at week 4. Long-Evans rats were used in the present study. Left ventricular samples(n=6) of both prediabetic and control groups were analyzed.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Rattus norvegicus

SUBMITTER: Imre Voros

PROVIDER: E-MTAB-8204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cardiac miRNA Expression and their mRNA Targets in a Rat Model of Prediabetes.

Sághy Éva É Vörös Imre I Ágg Bence B Kiss Bernadett B Koncsos Gábor G Varga Zoltán V ZV Görbe Anikó A Giricz Zoltán Z Schulz Rainer R Ferdinandy Péter P

International journal of molecular sciences 20200320 6

Little is known about the mechanism of prediabetes-induced cardiac dysfunction. Therefore, we aimed to explore key molecular changes with transcriptomic and bioinformatics approaches in a prediabetes model showing heart failure with preserved ejection fraction phenotype. To induce prediabetes, Long-Evans rats were fed a high-fat diet for 21 weeks and treated with a single low-dose streptozotocin at week 4. Small RNA-sequencing, in silico microRNA (miRNA)-mRNA target prediction, Gene Ontology ana ...[more]

PMID: 32244869

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