ArcZ comparative RIL-seq
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ABSTRACT: E. coli ΔarcZ hfq-Flag strain was transformed with a WT arcZ, mut arcZC81G or a mut arcZC81T,T82G,G83A plasmids. Single colonies of the transformants were grown overnight in LB at 37 °C with shaking (200 r.p.m.). Cultures were diluted 100-fold in fresh LB re-grown with shaking at 37 °C to an optical density of OD600 = 1.0 and induced with IPTG (1mM, 20 min), RIL-seq experiments were preformed as described in Melamed et al 2018, each repeated three times.
INSTRUMENT(S): NextSeq 500
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655
SUBMITTER: Raya Romm
PROVIDER: E-MTAB-8224 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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