Unknown,Transcriptomics,Genomics,Proteomics

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SuhB associates with Nus factors to facilitate 30S ribosome biogenesis in Escherichia coli


ABSTRACT: This experiment consists of two studies. The first where two independent biological replicates of MG1655, MG1655 thyA suhB::thyA and MG1655 thyA nusB::thyA were each grown in LB to mid-exponential phase. RNA-seq and associated data analysis were performed as described previously (Stringer et al., 2014. PMID 24272778). The second where five cultures of MG1655 thyA suhB::thyA were grown overnight from single colonies at 37 C in LB. 5 L of each overnight culture was spread on LB agar and incubated at 30 C, the non-permissive temperature for suhB mutants. One suppressor mutant colony was selected from each plate. rnc was PCR amplified from colonies using oligonucleotides JW836-JW837, and the PCR products were sequenced to identify the presence, if any, of suppressor mutations. Genomic DNA from a strain with wild-type rnc was prepared using a DNeasy Blood and Tissue Kit (Qiagen). A DNA library was prepared using a Nextera kit (Illumina). The library was sequenced (paired-end reads) using an Illumina MiSeq instrument.

INSTRUMENT(S): Illumina MiSeq, Illumina HiSeq 2000

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Navjot Singh 

PROVIDER: E-MTAB-4240 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in Escherichia coli.

Singh Navjot N   Bubunenko Mikhail M   Smith Carol C   Abbott David M DM   Stringer Anne M AM   Shi Ronald R   Court Donald L DL   Wade Joseph T JT  

mBio 20160315 2


<h4>Unlabelled</h4>A complex of highly conserved proteins consisting of NusB, NusE, NusA, and NusG is required for robust expression of rRNA in Escherichia coli. This complex is proposed to prevent Rho-dependent transcription termination by a process known as "antitermination." The mechanism of this antitermination in rRNA is poorly understood but requires association of NusB and NusE with a specific RNA sequence in rRNA known as BoxA. Here, we identify a novel member of the rRNA antitermination  ...[more]

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