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Sequencing of DNA captured with a methyl-CpG binding domain (MBD) of primary colorectal cancers, with paired normal tissue, and liver metastases from different patients.

ABSTRACT: We sought to understand the mechanisms of cancer dissemination and to improve treatments for late-stage cancer patients. Laser tissue microdissection of 10 to 25 x 106 µm2 epithelium from sections of freshly-frozen colorectal cancers (no=6), colorectal cancer liver metastases (no=12) and normal colonic mucosa (no=3) was performed to extract DNA. Methylated DNA was captured using a methylCpG binding domain (MBD) polypeptide and deep sequenced. We detected hyper and hypomethylation in both colorectal cancers and metastases compared to normal tissue.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Stephany Orjuela

PROVIDER: E-MTAB-8232 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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